chromogenic in situ hybridization rnascope  (Advanced Cell Diagnostics Inc)

Journal: Science Advances

Article Title: Mitochondrial-targeted antioxidant attenuates preeclampsia-like phenotypes induced by syncytiotrophoblast-specific Gαq signaling

doi: 10.1126/sciadv.adg8118

Figure Lengend Snippet: ( A ) Breeding paradigm for selective activation of the Gαq pathway in only the syncytiotrophoblast II layer. ( B ) General anatomy of the mouse placenta. ( C ) Double-transgenic placenta of hM3Dq F/F dam x Gcm1 -Cre +/− sire. Scale bars, 300 μm (left) and 10 μm (middle, right). ( D ) RNAscope-based in situ hybridization of double-transgenic placenta. Scale bars, 50 μm and 10 μm (inset on right). STB II, syncytiotrophoblast II layer. Images (A and B) were created using BioRender ( www.biorender.com ).

Article Snippet: Chromogenic duplex RNAscope-based in situ hybridization was performed on paraffin-embedded 4-μm placental sections (Advanced Cell Diagnostics, 322430).

Techniques: Activation Assay, Transgenic Assay, In Situ Hybridization

Journal: eBioMedicine

Article Title: B4GALNT3 regulates glycosylation of sclerostin and bone mass

doi: 10.1016/j.ebiom.2023.104546

Figure Lengend Snippet: B4galnt3 is expressed in osteoblasts and osteocytes but not in osteoclasts. a–l) Representative in situ hybridization images in mice. Transverse sections across lumbar vertebra 5 (a–d) and longitudinal sections of femur or tibia (e–l) in mice demonstrate the mRNA expression of B4galnt3 (blue; a–l) and Sost (red; a, e, i), Dmp1 (red; b, f, j), Runx2 (red; c, g, k) or Ctsk (red; d, h, l). B4galnt3 mRNA could be observed in Sost-, Dmp1-, and Runx2-expressing osteocytes and osteoblast- lineage cells on the bone surface. In contrast, B4galnt3 was not detectable in Ctsk-expressing osteoclasts. Scale bar 50 μm. Images are representative of at least three independent experiments.

Article Snippet: In situ hybridization The chromogenic in situ hybridization (cISH) was performed by using the RNAscope Duplex Detection Kit (322500, Advanced Cell Diagnostics (ACD), Bio-Techne Ltd., Abingdon, UK).

Techniques: In Situ Hybridization, Expressing